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KMID : 0545119990090040450
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 4 p.450 ~ p.456
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Anti-Complementary Properties of Polysaccharides Isolated from Fruit Bodies of Mushroom Pleurotus ostreatus
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Kweon, Mee Hyang
Jang, Hyo/Lim,Wang Jin/Chang, Hyo Ihl/Kim,Chan Wha/Yang, Han Chul/Hwang, Han Joon
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Abstract
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A high molecular-weight water-soluble fraction (PO) obtained by the ethanol precipitation of 0.1 N NaOH extracts of the mushroom Pleurotus ostreatus showed 82% anti-complementary activity for complement consumption hemolysis. The PO consisted of 42% carbohydrate (w/w), 50% protein (w/w), and 3% uronic acid (w/w). Fifty-eight percent of the anti-complementary activity decreased by periodate oxidation and 22% by protease digestion, suggesting that the sugar and protein moieties are essential for this activity. Two polysaccharide fractions, PO-Ⅲa-1 and POⅢa-2, with anti-complementary activity were isolated from the PO using DEAE-Sepharose FF followed by Sephadex G-75 and Sepharose CL-6B gel permeation chromatographies. The PO-Ⅲa-2 was found by HPLC to be nearly homogeneous, with the molecular mass of 531 kDa, and showed 96% ITCH50_50 (inhibition against the total complement hemolysis of deionized water as the control) at a concentration of 1 mg/ml. This fraction contained galactose, mannose, fucose, and glucose with molar ratios of 1.75:1:0.65 and 0.59, respectively. The majority of galactose and mannose units in the PO-Ⅲa-2 were composed of TGalp1→4,→6Galp1→, →2,6Galp1→, and →Manpl→. The PO-Ⅲa-1 (molecular mass of 2000 kDa), exhibiting higher activity than the PO-Ⅲa-2, was further purified into two fractions, unbound proteoglycan (PO-Ⅲa-1A) and bound glucomannan (PO-Ⅲa-1B), by affinity chromatography using ConA-Sepharose CL-4B. The anti-complementary activity of each affinity purified fraction decreased as compared to that of the native PO-Ⅲa-1 fraction, indicating that the formation of complex between both polysaccharide fractions was necessary for full anticomplementary activity.
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